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From: Steve on 3 Aug 2008 01:58
http://www.ncbi.nlm.nih.gov/pubmed/18577844?ordinalpos=53&itool=EntrezSystem
2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum

Tohoku J Exp Med. 2008 Jun;215(2):149-57. Links

Selective inhibition of cyclooxygenase-2 suppresses the growth of pancreatic
cancer cells in vitro and in vivo.

Xu XF, Xie CG, Wang XP, Liu J, Yu YC, Hu HL, Guo CY.
Department of Gastroenterology, Tenth Hospital, Tongji University, Shanghai,
China

Cyclooxygenase-2 (COX-2), a prostaglandin synthetase, is involved in
development of certain tumors. We therefore analyzed COX-2 expression in
pancreatic cancer tissues (53 samples) and Panc-1 human pancreatic cancer
cells by immunohistochemistry, RT-PCR and western-blotting analyses. Also,
immunohistochemistry of proliferating cell nuclear antigen (PCNA) was
performed. We found expression of COX-2 was dramatically upregulated in 36
of 53 cases (67.9%) and the expression of COX-2 was associated with the
diameter (> 3 cm) of the tumors (p < 0.05), but not with the age, gender,
tumor location, differentiation, lymph-node metastases and TNM stage. The
positivity rate of PCNA expression in the pancreatic cancer cells of the
COX-2 positive group (32.88 +/- 13.26%) was significantly higher than that
in the COX-2 negative group (24.56 +/- 11.51%) (p < 0.05). Then we
investigated the effect of selective inhibitors of COX-2 (NS398 and
celecoxib) on proliferation of Panc-1 cells by 3-(4,5
dimethyl-2-thiazolyl)-2.5-diphenyl-2H-tetrazolium bromide (MTT) assay.
Either NS398 or celecoxib suppressed proliferation of Panc-1 cells
dose-dependently in vitro. Furthermore, Panc-1 cells were implanted into
nude mice, and celecoxib was administrated orally with feed. The volume of
the tumor xenografted into nude mice was decreased by 51.6% in the celecoxib
group (p < 0.01). In conclusion, the increased expression of COX-2 may be
responsible for rapid proliferation of pancreatic cancer, and specific
inhibition of COX-2 suppresses proliferation of Panc-1 cells in vitro and in
nude mice. The selective inhibitor of COX-2 may be an effectual agent for
pancreatic cancer chemoprevention.



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