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From: Thomas Wagner on 16 Jun 2008 10:32
On Sat, 14 Jun 2008 06:33:44 +0100, Paul
<dontspamme(a)westgreen.freeserve.co.uk> wrote:

>And there I was thinking that if you get stinking drunk all the time,
>you could make the hep-c virus so intoxicated that it wouldn't be able
>to work and harm your liver.
>Oops !! Didn't spot it first time. That study was sponsored by
>Heineken.

Proteasome activation by hepatitis C core protein is reversed by
ethanol-induced oxidative stress.

Osna, Natalia A; White, Ronda L; Krutik, Viatcheslav M; Wang, Ting;
Weinman, Steven A; Donohue, Terrence M; Jr 2008-06-14
Background & Aims: The proteasome is a major cellular proteinase. Its
activity is modulated by cellular oxidants. Hepatitis C core protein and
ethanol exposure both cause enhanced oxidant generation. The aim was to
investigate whether core protein, by its ability to generate oxidants,
alters proteasome activity and whether these alterations are further
affected by ethanol exposure. Methods: These interactions were examined
in Huh-7 cell lines that expressed inducible HCV core protein and/or
constitutive cytochrome P450 2E1 (CYP2E1) and as purified components in
a cell-free system. Chymotrypsin-like proteasome activity was measured
fluorometrically. Results: Proteasome activity in core-positive 191-20
cells was 20% higher than that in core-negative cells and was enhanced
3-fold in CYP2E1-expressing L14 cells. Exposure of core-positive cells
to glutathione ethyl ester, catalase, or the CYP2E1 inhibitor diallyl
sulfide partially reversed the elevation of proteasome activity in
core-positive cells, whereas ethanol exposure suppressed proteasome
activity. The results indicate that proteasome activity was up-regulated
by low levels of core-induced oxidative stress but down-regulated by
high levels of ethanol-elicited stress. These findings were partially
mimicked in a cell-free system. Addition of core protein enhanced the
peptidase activity of purified 20S proteasome containing the proteasome
activator PA28 and was further potentiated by addition of liver
mitochondrial and/or microsome fractions. However, proteasome activation
was significantly attenuated when fractions were obtained from
ethanol-fed animals. Conclusions: HCV core protein interacts with PA28,
mitochondrial, and endoplasmic reticulum proteins to cause low levels of
oxidant stress and proteasome activation, which is dampened during
ethanol metabolism when oxidant generation is higher.
Gastroenterology
PubMed Database
--
To reach me, complete my last name in the address.
From: Waterspider on 12 Jul 2008 20:29

"TX-012" <withbacon(a)aol.com> wrote in message
news:19374208-2deb-40f8-ba34-9dc401726a14(a)t54g2000hsg.googlegroups.com...
> On Jun 12, 12:01 pm, "Cactus Jammies"
> <cactusjamm...(a)retinal.circus.orb> wrote:
>> different name same preoccupation
>> cactus jammies
>> just re-set my zapper
>>
>> "ironjustice" <teamtan...(a)hotmail.com> wrote
>
> I will never understand these people, the "the entire universe would
> be saved and all would be well if everyone only followed my one
> crackpot theory" internet kooks...
>
> There is a guy named Ernie Primeau who has been posting for year,
> countless times per day, on alt.baldspot and elsewhere trying to
> convince people that modern hairloss drugs cause baldness, and that
> the key to hair regrowth is to pluck all your body hair...
>
> There is a guy on nutrition newsgroups who insists that Jeebus would
> practically return and walk among us if only we consumed buttloads of
> coconut oil...
>
> Lloyd Wright and others will try to tell you that combo therapy for
> hep c is bad, bad, must be avoided, and that hep c can be cured
> "naturally"...
>
> And on and on and on...

Cripes, how many years now have we been trying to tell Ole Ironsides that
hepatitis c is not just another name for hemotomachrosis? He's prolly
smoking his leeches or sumpin...


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