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From: Elena Alifanov on 8 Mar 2005 16:59
I need some help with a homework assignment.

What do Western Blot and Elisa have in common and how do they differ?

Can someone suggest an answer or help me find one?

Thanks,
~Elena


From: Frank de Groot on 8 Mar 2005 18:15
> What do Western Blot and Elisa have in common and how do they differ?
>
> Can someone suggest an answer or help me find one?

Yup, STFW with Google.


From: Esteban Gabriel Vega on 20 Mar 2005 16:40
Well,
Western uses an agarose(or poliacrilamide) gel to separate proteins,and then
they're transfered to a nitrocelulose membrane so it allows you to compare
molecular wiegh . ELISA don't separate proteins, it uses a multiwell plate
where proteins are absorverd.
Both cases need an especific conjugated-antibody. In order to detect the
Ab-Ag reaction.
I don't know in terms of sensibility which is best; perhaps if you found
something you can tell me.

EV


From: Eric on 6 Apr 2005 02:20
Esteban Gabriel Vega wrote:
>
> Well,
> Western uses an agarose(or poliacrilamide) gel to separate proteins, and
> then they're transfered to a nitrocelulose membrane so it allows you to
> compare molecular wiegh . ELISA don't separate proteins, it uses a
> multiwell plate where proteins are absorverd.
> Both cases need an especific conjugated-antibody. In order to detect the
> Ab-Ag reaction.
> I don't know in terms of sensibility which is best; perhaps if you found
> something you can tell me.
>
> EV

A Western blot may provide some advantage in situations where your antibody
may cross react with other proteins. For example, since you can identify
your protein of interest by molecular weight, you can determine the activity
of the antibody against the protein vs background noise (non-specific
binding).

Eric
From: shamon on 14 Apr 2005 15:27
Eric <say.no(a)spam.now> wrote in message news:<42537FB5.7672D0B2(a)spam.now>...
> Esteban Gabriel Vega wrote:
> >
> > Well,
> > Western uses an agarose(or poliacrilamide) gel to separate proteins, and
> > then they're transfered to a nitrocelulose membrane so it allows you to
> > compare molecular wiegh . ELISA don't separate proteins, it uses a
> > multiwell plate where proteins are absorverd.
> > Both cases need an especific conjugated-antibody. In order to detect the
> > Ab-Ag reaction.
> > I don't know in terms of sensibility which is best; perhaps if you found
> > something you can tell me.
> >
> > EV
>
> A Western blot may provide some advantage in situations where your antibody
> may cross react with other proteins. For example, since you can identify
> your protein of interest by molecular weight, you can determine the activity
> of the antibody against the protein vs background noise (non-specific
> binding).
>
> Eric

the main advantage of the ELISA is that the protein is still intact ie
in its quaterniary / tertiary structure and so can be analysed from
both structural and functional integrity (eg with cell based assays).
whilst the western will denature you protein and only provide you with
limited information regarding its characteristics

shamon
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