Oxidative Stress-induced Insulin Resistance
in [Diabetes]
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From: ironjustice on 26 Sep 2008 13:46 Oxidative stress-induced insulin resistance in skeletal muscle cells is ameliorated by gamma-tocopherol treatment. Singh I, Carey AL, Watson N, Febbraio MA, Hawley JA Eur J Nutr 2008 Sep 18. BACKGROUND: Oxidative stress-induced reactive oxygen species are associated with the clinical manifestation of insulin resistance. Evidence suggests that antioxidant treatment may reduce this incidence. AIM OF THE STUDY: This study determined whether glucose oxidase (GO)-induced insulin resistance in cultured skeletal muscle cells could be ameliorated by pre-treatment with gamma-tocopherol (GT). METHODS: Insulin sensitivity in L6 myotubes was assessed by 2-deoxy-D: -[(3)H]- glucose uptake. The phosphorylation of distal insulin signaling proteins Akt and the Akt substrate AS160 were determined by western blot. RESULTS: One hour treatment with 100 mU/ml GO decreased insulin-stimulated glucose uptake (P < 0.001). Pre-treatment with GT either partially (100 microM) or completely (200 microM) restored insulin-stimulated glucose uptake in cells after GO- induced insulin resistance. GO-induced oxidative stress did not impair insulin stimulated phosphorylation of Akt or AS160, but 200 microM GT increased insulin- stimulated phosphorylation of these key signaling proteins (P < 0.05). CONCLUSIONS: High-dose (200 microM) GT treatment ameliorated oxidative stress- induced insulin resistance in cultured rat L6 skeletal muscle cells. European journal of nutrition [Eur J Nutr] Who loves ya. Tom Jesus Was A Vegetarian! http://tinyurl.com/2r2nkh Man Is A Herbivore! http://tinyurl.com/a3cc3 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk
From: anonymous on 26 Sep 2008 14:26 What causes insulin resistance that can lead to oxidative Stress ? Will you please in your own words explain this to us?
From: anonymous on 27 Sep 2008 10:57 What causes insulin resistance that can lead to oxidative Stress ? Will you please in your own words explain this to us?
From: anonymous on 27 Sep 2008 20:14 What causes insulin resistance that can lead to oxidative Stress ? Will you please in your own words explain this to us?
From: ironjustice on 27 Sep 2008 21:18
On Sep 27, 5:14 pm, anonym...(a)nowhere.you.know wrote: What causes insulin resistance that can lead to oxidative Stress ? Will you please in your own words explain this to us? << There are seven hundred causes of oxidative stress in diabetes .. Iron supplementation causes insulin resistance. Bloodletting cures insulin resistance. Iron deprivation cures insulin resistance. I wonder if it is iron .. ? Maybe we should do a large clinical trial of lowering iron levels in diabetes. We ARE doing a large clinical trial of iron depletion in diabetes. Now if you are STILL wondering .. what .. causes the oxidation in diabetes .. ? You are stupid .. and .. useless IN .. these .. sci .. groups. Logic .. Sooo .. next time .. IF .. and when the post goes to OTHER than a .. sci .. group .. YOU can post to it .. Because .. ? .. it will become a post which does NOT **exclusively** 'assume' .. you have at least something in your .. haid .. other than .. sht .. Who loves ya. Tom Jesus Was A Vegetarian! http://tinyurl.com/2r2nkh Man Is A Herbivore! http://tinyurl.com/a3cc3 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk |