Oxidative Stress and Food
in [Fibromyalgia]
|
From: ironjustice on 23 Jul 2008 17:42 "Unprecedented radioprotection by a dietary supplement comprised of ingredients available to the general public" Elevated DNA damage in a mouse model of oxidative stress: impacts of ionizing radiation and a protective dietary supplement. Lemon JA, Rollo CD, Boreham DR Mutagenesis 2008 Jul 21. Transgenic growth hormone (Tg) mice express elevated free radical processes and a progeroid syndrome of accelerated ageing. We examined bone marrow cells of Tg mice and their normal (Nr) siblings for three markers of DNA damage and assessed the impact of free radical stress using ionizing radiation. We also evaluated the radiation protection afforded by a dietary supplement that we previously demonstrated to extend longevity and reduce cognitive ageing of Nr and Tg mice. Spectral karyotyping revealed few spontaneous chromosomal aberrations in Nr or Tg. Tg mice, however, had significantly greater constitutive levels of both gammaH2AX and 8-hydroxy-deoxyguanosine (8-OHdG) compared to Nr. When exposed to a 2-Gy whole-body dose of ionizing radiation, both Nr and Tg mice showed significant increases in DNA damage. Compared to Nr mice, irradiated Tg mice had dramatically higher levels of gammaH2AX foci and double the levels of chromosomal aberrations. In unirradiated mice, the dietary supplement significantly reduced constitutive gammaH2AX and 8-OHdG in both Nr and Tg mice (normalizing both gammaH2AX and 8-OHdG in Tg), with little difference in gammaH2AX and 8-OHdG over constitutive levels. Induced chromosomal aberrations were also reduced, and in Nr mice, virtually absent. Remarkably, supplemented mice expressed 6-fold lower levels of radiation-induced chromosomal aberrations compared to unsupplemented Nr or Tg mice. Based on our data, the dietary supplement appeared to scavenge free radicals before they could cause damage. This study validates Tg mice as an exemplary model of oxidative stress and radiation hypersensitivity and documents unprecedented radioprotection by a dietary supplement comprised of ingredients available to the general public. -------------------------------------------------------------------------------- More from this journal Mutagenesis [Mutagenesis] Who loves ya. Tom Jesus Was A Vegetarian! http://tinyurl.com/2r2nkh Man Is A Herbivore! http://tinyurl.com/4rq595 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk
From: Taka on 24 Jul 2008 04:55 On Jul 24, 6:42 am, ironjustice <teamtan...(a)hotmail.com> wrote: > "Unprecedented radioprotection by a dietary supplement comprised of > ingredients available to the general public" > > Elevated DNA damage in a mouse model of oxidative stress: impacts of > ionizing radiation and a protective dietary supplement. Lemon JA, > Rollo CD, Boreham DR > Mutagenesis 2008 Jul 21. > > Transgenic growth hormone (Tg) mice express elevated free radical > processes and a progeroid syndrome of accelerated ageing. > We examined bone marrow cells of Tg mice and their normal (Nr) > siblings for three markers of DNA damage and assessed the impact of > free radical stress using ionizing radiation. > We also evaluated the radiation protection afforded by a dietary > supplement that we previously demonstrated to extend longevity and > reduce cognitive ageing of Nr and Tg mice. > Spectral karyotyping revealed few spontaneous chromosomal aberrations > in Nr or Tg. > Tg mice, however, had significantly greater constitutive levels of > both gammaH2AX and 8-hydroxy-deoxyguanosine (8-OHdG) compared to Nr. > When exposed to a 2-Gy whole-body dose of ionizing radiation, both Nr > and Tg mice showed significant increases in DNA damage. Compared to Nr > mice, irradiated Tg mice had dramatically higher levels of gammaH2AX > foci and double the levels of chromosomal aberrations. > In unirradiated mice, the dietary supplement significantly reduced > constitutive gammaH2AX and 8-OHdG in both Nr and Tg mice (normalizing > both gammaH2AX and 8-OHdG in Tg), with little difference in gammaH2AX > and 8-OHdG over constitutive levels. Induced chromosomal aberrations > were also reduced, and in Nr mice, virtually absent. > Remarkably, supplemented mice expressed 6-fold lower levels of > radiation-induced chromosomal aberrations compared to unsupplemented > Nr or Tg mice. > Based on our data, the dietary supplement appeared to scavenge free > radicals before they could cause damage. > This study validates Tg mice as an exemplary model of oxidative stress > and radiation hypersensitivity and documents unprecedented > radioprotection by a dietary supplement comprised of ingredients > available to the general public. > > -------------------------------------------------------------------------------- > More from this journal > Mutagenesis [Mutagenesis] > > Who loves ya. > Tom > > Jesus Was A Vegetarian!http://tinyurl.com/2r2nkh > > Man Is A Herbivore!http://tinyurl.com/4rq595 > > DEAD PEOPLE WALKINGhttp://tinyurl.com/zk9fk Cannot get to the full text :-( What is this mysterious dietary supplement? Don't say it's another iron chelator Tom ... Taka Mutagenesis. 2008 Jul 21. [Epub ahead of print] Radiation-induced apoptosis in mouse lymphocytes is modified by a complex dietary supplement: the effect of genotype and gender. Lemon JA, Rollo CD, McFarlane NM, Boreham DR. Department of Medical Physics and Applied Radiation Sciences. This study examined whether radiation sensitivity measured by lymphocyte apoptosis could be ameliorated by a complex anti-oxidant/ anti-ageing dietary supplement. We also examined lymphocytes from both genders of normal (Nr) mice as well as transgenic growth hormone (Tg) mice that express strongly elevated reactive oxygen species processes and a progeroid syndrome of accelerated ageing. We introduce Tg mice as a potentially valuable new model to study radiation sensitivity. Isolated lymphocytes from all experimental groups were exposed to gamma radiation and the time course of apoptosis was measured in vitro. Kinetics of radiation-induced apoptosis was similar among groups, which peaked at 8 h, but maximal levels differed significantly between groups. Nr male mice had 60% lower levels of radiation-induced apoptosis than Tg males, supporting our hypothesis that Tg mice would be radiation sensitive. The dietary supplement protected lymphocytes in male mice of both strains, with proportionally greater reductions in Tg mice. Lymphocytes from female mice (both Nr and Tg) were highly radiation resistant compared to males and the supplement provided no additional benefit at the doses used in this study. These results highlight that radiation-induced apoptosis is complex and is modified by genotype, dietary supplements and gender. PMID: 18644835 J Gerontol A Biol Sci Med Sci. 2005 Mar;60(3):275-9. A complex dietary supplement extends longevity of mice. Lemon JA, Boreham DR, Rollo CD. Department of Biology, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada L8S 4K1. Key factors implicated in aging include reactive oxygen species, inflammatory processes, insulin resistance, and mitochondrial dysfunction. All are exaggerated in transgenic growth hormone mice (TGM), which display a syndrome resembling accelerated aging. We formulated a complex dietary supplement containing 31 ingredients known to ameliorate all of the above features. We previously showed that this supplement completely abolished the severe age-related cognitive decline expressed by untreated TGM. Here we report that longevity of both TGM and normal mice is extended by this supplement. Treated TGM showed a 28% increase (p < .00008) in mean longevity. An 11% increase in mean longevity was also significant (p < .002093) for treated normal mice, compared to untreated normal mice. These data support the hypothesis that TGM are a model of accelerated aging, and demonstrate that complex dietary supplements may be effective in ameliorating aging or age-related pathologies where simpler formulations have generally failed. PMID: 15860460 Exp Biol Med (Maywood). 2003 Jul;228(7):800-10. A dietary supplement abolishes age-related cognitive decline in transgenic mice expressing elevated free radical processes. Lemon JA, Boreham DR, Rollo CD. Departments of Biology and Medical Physics and Applied Radiation Sciences Unit, McMaster University, Hamilton Ontario, Canada L8S 4K1. We previously found that transgenic mice overexpressing growth hormone (TGM) have elevated and progressively increasing free radical processes in brain that strongly correlates with reduced survivorship. Young mature TGM, however, displayed vastly enhanced learning of an eight-choice cued maze and qualitatively different learning curves than normal controls. Here we document the age-related patterns in learning ability of TGM and normal mice. Learning appeared inferior in both genotypes of very young mice but TGM were confirmed to be superior to normal mice upon maturity. Older TGM, however, showed rapid age-related loss of their exceptional learning, whereas normal mice at 1 year of age showed little change. The cognitive decline of TGM was abolished by a complex "anti-aging" dietary supplement formulated to promote membrane and mitochondrial integrity, increase insulin sensitivity, reduce reactive oxygen and nitrogen species, and ameliorate inflammation. Results are discussed in the context of reactive oxygen and nitrogen species, long-term potentiation, learning, aging and neuropathology, based on known impacts of the growth hormone axis on the brain, and characteristics of TGM. PMID: 12876299
From: Paul T. Holland on 24 Jul 2008 16:01 the answer to your question isn't in the [followup] study that tommy found - i happen to follow aging and cognition issues, so i remembered the original study in question - this is the part the researchers wrote about that really should have been mentioned: "Unfortunately, this mix of nutrients was developed for mice and isn't available for humans." it's from an earlier mouse study the same group did [iirc, back on 2005] - they compounded 31 separate ingredients to make a single supplement for the mouse study, to test out a theory http://biomed.gerontologyjournals.org/cgi/content/abstract/60/3/275 afaik, the actual ingredients list is only in the full text, which is usually only available from the 'for pay' journal sites. you're on your own to try to mix and match to make up your own blend...until some vit outfit markets it that is. - a while back, someone who had read the entire study came up with a partial list of ingr that are commercially available as equivalent to the studies list, but without human dosage levels appropriate for the mixture: Acetyl-L-carnitine/ acetyl-L-carnitine hydrochloride/ acetyl-L-carnitine arginate dihydrochloride R-dihydro-lipoic acid Fish oil� eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) Coenzyme Q10 DHEA Garlic Ginkgo biloba Ginseng L-glutathione with cysteine and vitamin C Melatonin Gamma E mixed tocopherols/sesame lignans Low-dose aspirin Digestive enzymes [??? not narrow enough ] Resveratrol the above is about half the lsit of what was in the study... hth paul Taka wrote: > > On Jul 24, 6:42 am, ironjustice <teamtan...(a)hotmail.com> wrote: > > "Unprecedented radioprotection by a dietary supplement comprised of > > ingredients available to the general public" > > > > Elevated DNA damage in a mouse model of oxidative stress: impacts of > > ionizing radiation and a protective dietary supplement. Lemon JA, > > Rollo CD, Boreham DR > > Mutagenesis 2008 Jul 21. > > > > Transgenic growth hormone (Tg) mice express elevated free radical > > processes and a progeroid syndrome of accelerated ageing. > > We examined bone marrow cells of Tg mice and their normal (Nr) > > siblings for three markers of DNA damage and assessed the impact of > > free radical stress using ionizing radiation. > > We also evaluated the radiation protection afforded by a dietary > > supplement that we previously demonstrated to extend longevity and > > reduce cognitive ageing of Nr and Tg mice. > > Spectral karyotyping revealed few spontaneous chromosomal aberrations > > in Nr or Tg. > > Tg mice, however, had significantly greater constitutive levels of > > both gammaH2AX and 8-hydroxy-deoxyguanosine (8-OHdG) compared to Nr. > > When exposed to a 2-Gy whole-body dose of ionizing radiation, both Nr > > and Tg mice showed significant increases in DNA damage. Compared to Nr > > mice, irradiated Tg mice had dramatically higher levels of gammaH2AX > > foci and double the levels of chromosomal aberrations. > > In unirradiated mice, the dietary supplement significantly reduced > > constitutive gammaH2AX and 8-OHdG in both Nr and Tg mice (normalizing > > both gammaH2AX and 8-OHdG in Tg), with little difference in gammaH2AX > > and 8-OHdG over constitutive levels. Induced chromosomal aberrations > > were also reduced, and in Nr mice, virtually absent. > > Remarkably, supplemented mice expressed 6-fold lower levels of > > radiation-induced chromosomal aberrations compared to unsupplemented > > Nr or Tg mice. > > Based on our data, the dietary supplement appeared to scavenge free > > radicals before they could cause damage. > > This study validates Tg mice as an exemplary model of oxidative stress > > and radiation hypersensitivity and documents unprecedented > > radioprotection by a dietary supplement comprised of ingredients > > available to the general public. > > > > -------------------------------------------------------------------------------- > > More from this journal > > Mutagenesis [Mutagenesis] > > > > Who loves ya. > > Tom > > > > Jesus Was A Vegetarian!http://tinyurl.com/2r2nkh > > > > Man Is A Herbivore!http://tinyurl.com/4rq595 > > > > DEAD PEOPLE WALKINGhttp://tinyurl.com/zk9fk > > Cannot get to the full text :-( What is this mysterious dietary > supplement? Don't say it's another iron chelator Tom ... > > Taka > > Mutagenesis. 2008 Jul 21. [Epub ahead of print] > > Radiation-induced apoptosis in mouse lymphocytes is modified by a > complex dietary supplement: the effect of genotype and gender. > > Lemon JA, Rollo CD, McFarlane NM, Boreham DR. > Department of Medical Physics and Applied Radiation Sciences. > > This study examined whether radiation sensitivity measured by > lymphocyte apoptosis could be ameliorated by a complex anti-oxidant/ > anti-ageing dietary supplement. We also examined lymphocytes from both > genders of normal (Nr) mice as well as transgenic growth hormone (Tg) > mice that express strongly elevated reactive oxygen species processes > and a progeroid syndrome of accelerated ageing. We introduce Tg mice > as a potentially valuable new model to study radiation sensitivity. > Isolated lymphocytes from all experimental groups were exposed to > gamma radiation and the time course of apoptosis was measured in > vitro. Kinetics of radiation-induced apoptosis was similar among > groups, which peaked at 8 h, but maximal levels differed significantly > between groups. Nr male mice had 60% lower levels of radiation-induced > apoptosis than Tg males, supporting our hypothesis that Tg mice would > be radiation sensitive. The dietary supplement protected lymphocytes > in male mice of both strains, with proportionally greater reductions > in Tg mice. Lymphocytes from female mice (both Nr and Tg) were highly > radiation resistant compared to males and the supplement provided no > additional benefit at the doses used in this study. These results > highlight that radiation-induced apoptosis is complex and is modified > by genotype, dietary supplements and gender. > PMID: 18644835 > > J Gerontol A Biol Sci Med Sci. 2005 Mar;60(3):275-9. > > A complex dietary supplement extends longevity of mice. > > Lemon JA, Boreham DR, Rollo CD. > Department of Biology, McMaster University, 1280 Main Street West, > Hamilton, Ontario, Canada L8S 4K1. > > Key factors implicated in aging include reactive oxygen species, > inflammatory processes, insulin resistance, and mitochondrial > dysfunction. All are exaggerated in transgenic growth hormone mice > (TGM), which display a syndrome resembling accelerated aging. We > formulated a complex dietary supplement containing 31 ingredients > known to ameliorate all of the above features. We previously showed > that this supplement completely abolished the severe age-related > cognitive decline expressed by untreated TGM. Here we report that > longevity of both TGM and normal mice is extended by this supplement. > Treated TGM showed a 28% increase (p < .00008) in mean longevity. An > 11% increase in mean longevity was also significant (p < .002093) for > treated normal mice, compared to untreated normal mice. These data > support the hypothesis that TGM are a model of accelerated aging, and > demonstrate that complex dietary supplements may be effective in > ameliorating aging or age-related pathologies where simpler > formulations have generally failed. > PMID: 15860460 > > Exp Biol Med (Maywood). 2003 Jul;228(7):800-10. > > A dietary supplement abolishes age-related cognitive decline in > transgenic mice expressing elevated free radical processes. > > Lemon JA, Boreham DR, Rollo CD. > Departments of Biology and Medical Physics and Applied Radiation > Sciences Unit, McMaster University, Hamilton Ontario, Canada L8S 4K1. > > We previously found that transgenic mice overexpressing growth hormone > (TGM) have elevated and progressively increasing free radical > processes in brain that strongly correlates with reduced survivorship. > Young mature TGM, however, displayed vastly enhanced learning of an > eight-choice cued maze and qualitatively different learning curves > than normal controls. Here we document the age-related patterns in > learning ability of TGM and normal mice. Learning appeared inferior in > both genotypes of very young mice but TGM were confirmed to be > superior to normal mice upon maturity. Older TGM, however, showed > rapid age-related loss of their exceptional learning, whereas normal > mice at 1 year of age showed little change. The cognitive decline of > TGM was abolished by a complex "anti-aging" dietary supplement > formulated to promote membrane and mitochondrial integrity, increase > insulin sensitivity, reduce reactive oxygen and nitrogen species, and > ameliorate inflammation. Results are discussed in the context of > reactive oxygen and nitrogen species, long-term potentiation, > learning, aging and neuropathology, based on known impacts of the > growth hormone axis on the brain, and characteristics of TGM. > PMID: 12876299
From: ironjustice on 24 Jul 2008 17:47 "Unprecedented radioprotection by a dietary supplement comprised of ingredients available to the general public" Elevated DNA damage in a mouse model of oxidative stress: impacts of ionizing radiation and a protective dietary supplement. Lemon JA, Rollo CD, Boreham DR Mutagenesis 2008 Jul 21. Transgenic growth hormone (Tg) mice express elevated free radical processes and a progeroid syndrome of accelerated ageing. We examined bone marrow cells of Tg mice and their normal (Nr) siblings for three markers of DNA damage and assessed the impact of free radical stress using ionizing radiation. We also evaluated the radiation protection afforded by a dietary supplement that we previously demonstrated to extend longevity and reduce cognitive ageing of Nr and Tg mice. Spectral karyotyping revealed few spontaneous chromosomal aberrations in Nr or Tg. Tg mice, however, had significantly greater constitutive levels of both gammaH2AX and 8-hydroxy-deoxyguanosine (8-OHdG) compared to Nr. When exposed to a 2-Gy whole-body dose of ionizing radiation, both Nr and Tg mice showed significant increases in DNA damage. Compared to Nr mice, irradiated Tg mice had dramatically higher levels of gammaH2AX foci and double the levels of chromosomal aberrations. In unirradiated mice, the dietary supplement significantly reduced constitutive gammaH2AX and 8-OHdG in both Nr and Tg mice (normalizing both gammaH2AX and 8-OHdG in Tg), with little difference in gammaH2AX and 8-OHdG over constitutive levels. Induced chromosomal aberrations were also reduced, and in Nr mice, virtually absent. Remarkably, supplemented mice expressed 6-fold lower levels of radiation-induced chromosomal aberrations compared to unsupplemented Nr or Tg mice. Based on our data, the dietary supplement appeared to scavenge free radicals before they could cause damage. This study validates Tg mice as an exemplary model of oxidative stress and radiation hypersensitivity and documents unprecedented radioprotection by a dietary supplement comprised of ingredients available to the general public. -------------------------------------------------------------------------------- More from this journal Mutagenesis [Mutagenesis] Who loves ya. Tom Jesus Was A Vegetarian! http://tinyurl.com/2r2nkh Man Is A Herbivore! http://tinyurl.com/4rq595 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk
From: ironjustice on 24 Jul 2008 21:25 "Unprecedented radioprotection by a dietary supplement comprised of ingredients available to the general public" Elevated DNA damage in a mouse model of oxidative stress: impacts of ionizing radiation and a protective dietary supplement. Lemon JA, Rollo CD, Boreham DR Mutagenesis 2008 Jul 21. Transgenic growth hormone (Tg) mice express elevated free radical processes and a progeroid syndrome of accelerated ageing. We examined bone marrow cells of Tg mice and their normal (Nr) siblings for three markers of DNA damage and assessed the impact of free radical stress using ionizing radiation. We also evaluated the radiation protection afforded by a dietary supplement that we previously demonstrated to extend longevity and reduce cognitive ageing of Nr and Tg mice. Spectral karyotyping revealed few spontaneous chromosomal aberrations in Nr or Tg. Tg mice, however, had significantly greater constitutive levels of both gammaH2AX and 8-hydroxy-deoxyguanosine (8-OHdG) compared to Nr. When exposed to a 2-Gy whole-body dose of ionizing radiation, both Nr and Tg mice showed significant increases in DNA damage. Compared to Nr mice, irradiated Tg mice had dramatically higher levels of gammaH2AX foci and double the levels of chromosomal aberrations. In unirradiated mice, the dietary supplement significantly reduced constitutive gammaH2AX and 8-OHdG in both Nr and Tg mice (normalizing both gammaH2AX and 8-OHdG in Tg), with little difference in gammaH2AX and 8-OHdG over constitutive levels. Induced chromosomal aberrations were also reduced, and in Nr mice, virtually absent. Remarkably, supplemented mice expressed 6-fold lower levels of radiation-induced chromosomal aberrations compared to unsupplemented Nr or Tg mice. Based on our data, the dietary supplement appeared to scavenge free radicals before they could cause damage. This study validates Tg mice as an exemplary model of oxidative stress and radiation hypersensitivity and documents unprecedented radioprotection by a dietary supplement comprised of ingredients available to the general public. -------------------------------------------------------------------------------- More from this journal Mutagenesis [Mutagenesis] Who loves ya. Tom Jesus Was A Vegetarian! http://tinyurl.com/2r2nkh Man Is A Herbivore! http://tinyurl.com/4rq595 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk
|
Next
|
Last
Pages: 1 2 Prev: Lefty you were told to stay off my threads Next: homophobe tries to deny his words |