Oxidative Stress In Lupus
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From: ironjustice on 23 Jul 2008 07:36 On Jul 23, 2:11 am, Chuck <ShorThi...(a)aol.com> wrote: Wow, oxidative stress plays a role in pathological damage. What a revelation! Whodathunkit Arealshocka << A real shocker to YOU .. mr .. "nutritionist" .. Chuck .. what did I tell you about my threads .. ? Take the advice .. NOBODY wants to hear from a hasbeen .. buddy .. Can you .. understand .. ? Who loves ya. Tom Jesus Was A Vegetarian! http://tinyurl.com/2r2nkh Man Is A Herbivore! http://tinyurl.com/4rq595 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk
From: Chuck on 23 Jul 2008 09:05 On Jul 23, 7:36�am, ironjustice <teamtan...(a)hotmail.com> wrote: > Chuck .. what did I tell you about my threads .. ? "Your" threads? Ever have the inflammatory / oxdation levels of your brain tissue tested? > > NOBODY wants to hear from a hasbeen .. buddy .. > > Can you .. understand .. ? While you cut and paste all-day, I'm doing real writing, research, and conference presentations on the subject. You've probably cut and pasted some of my work. Get some help, guy. Seriously. CB
From: ironjustice on 23 Jul 2008 14:28 On Jul 23, 6:05 am, Chuck <ShorThi...(a)aol.com> wrote:Get some help, guy. Seriously. << What part .. OF .. stay off my threads don't ya understand .. chuck .. you pathetic fkg hasbeen .. ? What .. part .. Who loves ya. Tom Jesus Was A Vegetarian! http://tinyurl.com/2r2nkh Man Is A Herbivore! http://tinyurl.com/4rq595 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk
From: Paul T. Holland on 23 Jul 2008 19:00 ironjustice wrote: > > On Jul 23, 2:11 am, Chuck <ShorThi...(a)aol.com> wrote: > Wow, oxidative stress plays a role in pathological damage. What a > revelation! Whodathunkit Arealshocka << > > A real shocker to YOU .. mr .. "nutritionist" .. that you would post such? > > Chuck .. what did I tell you about my threads .. ? not much - just some silliness about you having the right to decide who can post - no pays any attention but go ahead and try to demand some more > > Take the advice .. no > > NOBODY ob quirk - you are singular in your objection - please discontinue speaking for others >wants to hear from a hasbeen .. buddy .. lol > > Can you .. understand .. ? > not doubt the op does - you however, have proven that you don't > Who loves ya. > Tom > > Jesus Was A Vegetarian! > http://tinyurl.com/2r2nkh > > Man Is A Herbivore! > http://tinyurl.com/4rq595 > > DEAD PEOPLE WALKING > http://tinyurl.com/zk9fk
From: ironjustice on 24 Jul 2008 07:06
"Oxidative stress damage in systemic lupus erythematosus " "Premature onset of atherogenesis" Detection of Catalase as a major protein target of the lipid peroxidation product 4-HNE and the lack of its genetic association as a risk factor in SLE. D'Souza A, Kurien BT, Rodgers R, Shenoi J, Kurono S, Matsumoto H, Hensley K, Nath SK, Scofield RH BMC Med Genet 2008 Jul 7; 9(1):62. ABSTRACT: BACKGROUND: Systemic lupus erythematosus (SLE) is a multifactorial disorder characterized by the presence of autoantibodies. We and others have implicated free radical mediated peroxidative damage in the pathogenesis of SLE. Since harmful free radical products are formed during this oxidative process, including 4-Hydroxy 2-nonenol (4- HNE) and malondialdehyde (MDA), we hypothesized that specific HNE- protein adducts would be present in SLE red blood cell (RBC) membranes. Catalase is located on chromosome 11p13 where linkage analysis has revealed a marker in the same region of the genome among families with thrombocytopenia, a clinical manifestation associated with severe lupus in SLE affected pedigrees. Moreover, SLE afflicts African-Americans three times more frequently than their European American counterparts. Hence we investigated the effects of a genetic polymorphism of catalase on risk and severity of SLE in 48 pedigrees with African American ancestry. METHODS: Tryptic digestion followed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) analysis was used to identify the protein(s) modified by HNE, following Coomassie staining to visualize the bands on the acrylamide gels. Genotyping analysis for the Ci T, -262bp polymorphism in the promoter region of catalase was performed by PCR-RFLP and direct PCR- sequencing. We used a apedigree disequilibrium testa for the family based association analysis, implemented in the PDT program to analyze the genotyping results. RESULTS: We found two proteins to be HNE-modified, migrating around 80 and 50 kD respectively. Tryptic digestion followed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI- TOFMS) analysis of the Coomassie stained 80 kD band revealed that the target of HNE modification was catalase, a protein shown to associate with RBC membrane proteins. All the test statistics carried out on the genotyping analysis for the Ci T, -262bp polymorphism in the promoter region of catalase were non-significant (p>0.05) in our data, which suggested that this SNP is not associated with SLE. CONCLUSION: Our results indicate that catalase is one of the proteins modified due to oxidative stress. However, catalase may not be a susceptibility gene for SLE. Nonetheless, catalase is oxidatively modified among SLE patients. This suggests a possible role between oxidative modification of catalase and its affects on enzymatic activity in SLE. An oxidatively modified catalase could be one of the reasons for lower enzymatic activity among SLE subjects, which in turn could favor the accumulation of deleterious hydrogen peroxide. Furthermore, HNE-products are potential neoantigens and could be involved in the pathogenesis of SLE. Decrease in catalase activity could affect the oxidant-antioxidant balance. Chronic disturbance of this balance in patients with SLE may work favorably for the premature onset of atherogenesis with severe vascular effect. BMC medical genetics [BMC Med Genet] -------------------------------------------------------------------------------- Who loves ya. Tom Jesus Was A Vegetarian! http://tinyurl.com/2r2nkh Man Is A Herbivore! http://tinyurl.com/4rq595 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk |