Immunology

ELISA problems ('dead wells')
Hi, I'm experiencing a new (to me) problem with ELISA. I have an double sandwich human IgA ELISA using HRP (and TMB as the substrate) using Greiner Medium bind plates. The standard curve is good. I have had some problems with poor CV% on some runs and we are now seeing 'dead well' - wells that just don't ... 24 Feb 2006 11:14

urinalysis-Cipro for enterococcus?
Does anybody understand what these susceptibility results mean for an enterococcus UTI? (reported in MICs) Cipro S=1 Levofloxavin S<=1 Nitrofurantoin S<=32 Penicillin S=2 Vancomycin S<=0.5 ... 14 Feb 2006 10:46

Vitamin C & Infertility?
Hello, Do Vitamin C and Zinc supplementation is related to increase in Sperm counts, motility an viability and infertility? if yes, how? Can deficiency of these cause sperms maturation or meturation arrest at spermatocyte stage and motility of sperms? Is it due to imbalanced pH in testis? http://www.cforyou... 12 Jan 2006 22:54

Cancer Cure?
"Cancer Causes Tumors are caused by mutations in DNA of cells. An accumulation of mutations is needed for a tumor to emerge. Mutations that activate oncogenes or repress tumor supressor genes can eventually lead to tumors. Cells have mechanisms that repair DNA and other mechanisms that cause the cell to destroy ... 19 Dec 2005 14:45

Conventional Medicines-Can we become resistant?
Helo, Effects from Natural and unnantural substances to us/our body are two type of substance which is commonly thought. Natural means here: "those substances of which we have inherent sense of right and wrong" not naturally produced or in natural form. Since our evolution till today, our body have evolved or ... 22 Dec 2005 15:10

purpura
Hello In January 28th of 2005 i was hospitalised with ideopatic (or autoimmune) trombocitopenic purpura. Someone can tell me what are the causes of this disease? Thanks Ana M. M. ... 3 Dec 2005 11:17

Questions on Immunity & auto-immunity?
Hello, What tells us pathologically, whether we are immuno-deficient or immuno-strong? Are healthy or weak RBCs, WBCs related to it? Can circulating old senile worn out cells trigger auto-immune response? I am asking these questions in view of following notes as given in one book relating to some alt. system... 3 Dec 2005 11:17

Hives
Hello immune people: I have a problem with hives that has been going on for about a year. (Comes & goes, many different spots, rubbing/chafing seems to aggravate it.) I can't figure out what I may be allergic to. I'm not sure if my immune system is going strange. I am a 45 yr old woman, had hysterectomy 2 yrs ago... 7 Oct 2005 21:03

Vaccinations for India
I am travelling to India on May 1 and have been warned by everyone to get vaccianted for Hep A, Hep B, and typhoid. My insurance doesn't cover such vaccinations. I am only going for a week and staying in the cities in business-class hotels. My questions are: 1) Are these vaccinations really necessary? 2) Wh... 7 Oct 2005 21:03

Western Blot vs. Elisa?
I need some help with a homework assignment. What do Western Blot and Elisa have in common and how do they differ? Can someone suggest an answer or help me find one? Thanks, ~Elena ... 7 Oct 2005 21:03